To investigate the effect of 38% SDF and its serial dilutions on the Stem cells from Human Exfoliated Deciduous teeth (SHED) and its ability to release growth factors from deciduous dentine.
The viability of SHED post-exposure to 38%, 3.8%, 0.38%, 0.038%, and 0.0038% SDF were assessed at 2, 5, and 7 days using the CyQuant assay, and results were validated using the MTT assay. The osteogenic differentiation of the cells was also investigated post-exposure to 0.0038% SDF. The release of the growth factors; TGF-β1, FGF-b, BMP-2, and VEGF from deciduous dentin discs exposed to 38% SDF, 0.0038% SDF, Ca(OH)2, MTA, and 17% EDTA were examined using ELISA. Statistical analysis was performed using means and standard deviations (p < 0.05). Two-way ANOVA compared the means of more than two groups with Tukey’s multiple comparison test. The unpaired t-test was also used to compare the differences between the two data sets.
In the presence of SDF concentrations of 38%, 3.8%, and 0.38%, SHED were not viable, 0.038% SDF reduced the cell viability, and 0.0038% SDF enhanced the cellular proliferation at day 5 of exposure. The growth factors released from deciduous dentin in response to 38% SDF and 0.0038% SDF were not statistically significantly different for TGF-β1, BMP-2, and FGF. While VEGF showed a significantly higher release with 0.0038% SDF.
38% SDF released dentinogenic growth factors from dentin discs, potentially explaining its role in reactionary dentinogenesis. Moreover, 0.0038% SDF resulted in a non-cytotoxic concentration that enhanced cellular proliferation and released bioactive molecules from dentin comparable to the 38% concentration. After further investigations, the 0.0038% dilution of SDF could present itself as a clinical concentration.
Harvard: M.M. Khalil, A. Bashir, Y. Monjazebi, M. Shah, A.R. Tawfik, I. Hussein, M. Jamal (2023) "Silver diamine fluoride: A stem cell cytotoxicity and dentin growth factor release in-vitro study", European Journal of Paediatric Dentistry, (), pp1-. doi: 10.23804/ejpd.2023.1928
Copyright (c) 2021 Ariesdue
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.