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Home Biological effects of sodium fluoride varnishes used in remineralisation of enamel: An...

Biological effects of sodium fluoride varnishes used in remineralisation of enamel: An in vitro study

Authors:

  • D. M. Escobar-García
    Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, University of San Luis Potosi, Mexico
  • J. Puente-Amaro
    Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, University of San Luis Potosi, Mexico
  • M. Á Rosales-Berber
    Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, University of San Luis Potosi, Mexico
  • A. Pozos-Guillén
    Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, University of San Luis Potosi, Mexico
  • S. Ruiz-Rodríguez
    Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, University of San Luis Potosi, Mexico
  • A. Garrocho-Rangel
    Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, University of San Luis Potosi, Mexico

DOI:

https://doi.org/10.23804/ejpd.2021.22.02.4

ABSTRACT


Material and methods

Four experimental assays were carried out (MTS, Mitotracker® system [mitochondrial function and morphology], Live/Dead®, and Comet) to assess the morphology, viability, and genotoxicity of two NaF varnishes (Duraphat® and Clinpro White®, both at two different concentrations). The essays were conducted on cultured pulp fibroblasts, grouped in four experimental and two control groups. Collected data were analysed by one-way ANOVA followed by the post hoc Bonferroni test.

Results

Some morphological changes of DPFs could be detected after the NaFVs stimulation. Most DPFs incubated in Duraphat (22.6 mg/L) maintained their morphological characteristics, except for a small decrease in cell size and shorter cytoplasmic projections (filopodia); DPFs treated with Clinpro White Varnish (22.6 mg/L) presented a morphology and size similar to the control group. DPFs exposed to Duraphat (113 mg/L) exhibited significant morphological alterations with considerable cell size increases and DPFs treated with Clinpro White Varnish (113 mg/L) showed a slight cell size increase without noticeable morphological anomalies. The Duraphat (22.6 mg/L) and Clinpro White Varnish (22.6 mg/L) groups promoted 31% and 35% cell proliferation, respectively, whereas DPFs proliferation with Duraphat (113 mg/L) decreased up to 59%, and cell proliferation with Clinpro White Varnish (113 mg/L) was similar to that of control.

Conclusion

All tested varnishes induced changes in the fibroblastic mitochondria. In general, Duraphat was less biocompatible and caused a change in the number of mitochondria compared to Clinpro White Varnish.

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Publication date:

June /2021

Issue:

Vol.22 – n.2/2021

Page:

107 – 113

Publisher:

Ariesdue

Topic:

Prevention and lifestyle

Cite:


Harvard: D. M. Escobar-García, J. Puente-Amaro, M. Á Rosales-Berber, A. Pozos-Guillén, S. Ruiz-Rodríguez, A. Garrocho-Rangel (2021) "Biological effects of sodium fluoride varnishes used in remineralisation of enamel: An in vitro study", European Journal of Paediatric Dentistry, 22(2), pp107-113. doi: 10.23804/ejpd.2021.22.02.4
Vancouver: D. M. Escobar-García, J. Puente-Amaro, M. Á Rosales-Berber, A. Pozos-Guillén, S. Ruiz-Rodríguez, A. Garrocho-Rangel. Biological effects of sodium fluoride varnishes used in remineralisation of enamel: An in vitro study. European Journal of Paediatric Dentistry [Internet]. 2021Jun.1 [cited 2023Mar.25];22(2):107-113. Available from: https://www.ejpd.eu/abstract-pubmed/biological-effects-of-sodium-fluoride-varnishes-used-in-remineralisation-of-enamel-an-in-vitro-study/
MLA: D. M. Escobar-García, J. Puente-Amaro, M. Á Rosales-Berber, A. Pozos-Guillén, S. Ruiz-Rodríguez, A. Garrocho-Rangel Biological effects of sodium fluoride varnishes used in remineralisation of enamel: An in vitro study. European Journal of Paediatric Dentistry. 2021;22(2):107-113

Copyright (c) 2021 Ariesdue

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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    European Journal of Paediatric Dentistry © | ISSN (Online): 2035-648X
    Registrazione del Tribunale di Milano n. 285 del 14.04.1998 | ROC 1946 - 26.09.2001
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