AIM To compare early bacterial adhesion and biofilm formation in vitro by different oral streptococci on a variety of
METHODS Adhesion and biofilm formation in vitro of 6 Streptococcus spp. on 15 different commercial brackets, in standard
culture medium and in human saliva were evaluated by the MTT reduction assay.
RESULTS Significant differences were evidenced in both early adhesion and biofilm formation among the studied brackets
and between the two conditions of growth. Gold brackets resulted less prone to colonisation compared to composite
brackets. The growth rates of the tested species on the different tested materials were significantly different.
CONCLUSION The adopted experimental plan, dissecting the two phases of plaque formation on different brackets in
different conditions, showed that composite brackets are more susceptible to adhesion and colonisation by streptococci,
while the remaining tested brackets did not show differences that could be clinically relevant. Data suggest that different
personal behaviours affecting the oral environment could significantly affect colonisation of brackets by oral
Vol.14 – n.2/2013
Harvard: C. Passariello, P. Gigola (2013) "Adhesion and biofilm formation by oral streptococci on different commercial brackets", European Journal of Paediatric Dentistry, 14(2), pp125-130. doi:
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